Journal article
Identification of copy number alterations associated with the progression of DCIS to invasive ductal carcinoma
CE Johnson, KL Gorringe, ER Thompson, K Opeskin, SE Boyle, Y Wang, P Hill, GB Mann, IG Campbell
Breast Cancer Research and Treatment | Published : 2012
Abstract
Ductal carcinoma in situ (DCIS) is a nonobligate precursor to invasive ductal carcinoma (IDC). Annotation of the genetic differences between the two lesions may assist in the identification of genes that promote the invasive phenotype. Synchronous DCIS and IDC cells were microdissected from FFPE tissue and analysed by molecular inversion probe (MIP) copy number arrays. Matched IDC and DCIS showed highly similar copy number profiles (average of 83% of the genome shared) indicating a common clonal origin although there is evidence that the DCIS continues to evolve in parallel with the co-existing IDC. Four chromosomal regions of loss (3q, 6q, 8p and 11q) and four regions of gain (5q, 16p, 19q ..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
The authors would like to dedicate this publication to the memory of Clint Johnson, who carried out many of the experiments as part of his PhD candidature. He was a fine scholar, talented scientist-in-training, and is sadly missed by his colleagues. His PhD candidature was supported by an Australian Postgraduate Award. This study was also supported by the Victorian Breast Cancer Research Consortium and the Australian National Health and Medical Research Council (NHMRC, No. 509050).